DOI: 10.36016/VM-2020-106-16

Veterinary Medicine: inter-departmental subject scientific collection. 2020. Issue 106. P. 87–93.

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IMMUNOLOGICAL EVALUATION OF THE APPLICATION OF EXPERIMENTAL VACCINES “REPRO-SUI-VAC-P” IN INDUSTRIAL PIG BREEDING

Buzun А. І. ¹, Коlchyk О. V. ¹, Borovkova V. M. ², Bobrovytska І. А. ¹

¹ National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine, e-mail: epibuz@ukr.net

² Kharkiv Zooveterinary Academy, Kharkiv, Ukraine

The article presents data on the optimization of the clinical protocol for the use of oil and adsorbed forms of experimental vaccine “ReproSuiVac-P” in industrial pig breeding (in the nucleus of herd, in groups of growing and fattening) in the system of biosafety measures “StopASFmix” based on immunological indicators of immunity, including humoral and cellular, with regards of the average daily growth of piglets) and specific (dynamics of postvaccinal antibodies, the intensity of population immunity, the resistance of vaccinated pigs relative to carrier-status of Mannheimia haemolytica). The obtained results allowed, at the level of probability not less than p≤0.01 (n = 879 pigs of different technological groups), to develop a technological card of vaccinations, which provides application of: a) oil form “ReproSuiVac-P” with bacterin on boars, lactating and barren sows twice with an interval 3 weeks in doses of 5+5 sm³; b) oil form of the vaccine in the group of weaned piglets 32^nd–35^th days of age at a single dose of 1–2 sm³; c) adsorbed with toxoid form of vaccine to sows of the second trimester of gestation twice with an interval of 2 weeks in doses of 5+7 sm³. Based on the indicators of constitutional and specific post-vaccination immunity against associated circovirus-pasteurellosis infection, a technological card of application of experimental vaccine “ReproSuiVac-P” in the main herd and in rearing groups of the industrial pig breeding was developed

Keywords: circovirus-pasteurellosis infection, post-vaccination immunity, constitutional immunity, specific immunity

References

Thrusfield M. et al. Veterinary epidemiology. 4^th ed. Hoboken, NJ: Wiley, 2018. 896 pp. DOI: https://doi.org/10.1002/9781118280249.

Ouyang T. et al. Co-infection of swine with porcine circovirus type 2 and other swine viruses. Viruses. 2019. Vol. 11, No. 2. P. 185. DOI: https://doi.org/10.3390/v11020185.

Buzun A.-I. Development of “StopASFmix” biosafety system for commodity piggery of Ukraine. Proceedings of the I International Scientific and Theoretical Conference “Interdisciplinary Research: Scientific Horizons and Perspectives”, Vilnius, March 12, 2021. Vilnus, 2021. Vol. 2. P. 9–14. DOI: https://doi.org/10.36074/scientia-12.03.2021.

Ran X. et al. Generation of porcine Pasteurella multocida ghost vaccine and examination of its immunogenicity against virulent challenge in mice. Microbial Pathogenesis. 2019. Vol. 132. P. 208–214. DOI: https://doi.org/10.1016/j.micpath.2019.04.016.

Hemann M. et al. Vaccination with inactivated or live-attenuated chimeric PCV1–2 results in decreased viremia in challenge-exposed pigs and may reduce transmission of PCV2. Veterinary Microbiology. 2012. Vol. 158, No. 1–2. P. 180–186. DOI: https://doi.org/10.1016/j.vetmic.2012.01.024.

Foerster T. et al. An inactivated whole-virus porcine parvovirus vaccine protects pigs against disease but does not prevent virus shedding even after homologous virus challenge. The Journal of General Virology. 2016. Vol. 97, No. 6. P. 1408–1413. DOI: https://doi.org/10.1099/jgv.0.000446.

Бузун А. І. та ін. Вакцина проти репродуктивно-респіраторного синдрому цирковірусної та парвовірусної інфекції свиней асоційована інактивована емульсована: патент 122732 Україна. № u201707521; заявл. 17.07.17; опубл. 25.01.18, бюл. № 2. 4 с.

Бузун А. І. та ін. Вакцина «Репросуівак-П» проти асоційованих інфекцій свиней (цирковірусна інфекція, репродуктивно-респіраторний синдром та пастерельоз свиней): патент 136316 Україна. № u201902406; заявл. 11.03.19; опубл. 12.08.19, бюл. № 15. 2 с.

OIE (World Organisation for Animal Health). Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). 8^th ed. Paris: OIE, 2018. 1833 pp.

Whitbread T. J. Clinical biochemistry (last full review/revision Jun 2015). URL: https://www.msdvetmanual.com/clinical-pathology-and-procedures/diagnostic-procedures-for-the-private-practice-laboratory/clinical-biochemistry.

Helke K. L. et al. Biology and diseases of swine. In: Fox J. G. et al. (eds) Laboratory Animal Medicine. 3^rd ed. Oxford: Academic Press, 2015. P. 695–769. DOI: https://doi.org/10.1016/B978-0-12-409527-4.00016-X.