DOI: 10.36016/VM-2020-106-13

Veterinary Medicine: inter-departmental subject scientific collection. 2020. Issue 106. P. 73–77.

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SELECTION OF OLIGONUCLEOTIDE SEQUENCES FOR THE PURPOSE OF DETECTION OF GENETIC MATERIAL OF CHLAMYDIA SPP. BY THE REACTION OF AMPLIFICATION

Pavlov S. L., Stegniy B. T.

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine, e-mail: psl600@i.ua

The article presents the results of bioinformatic analysis of 112 16s-23s rRNA operon sequences of different chlamydia species with the aim of conserved regions selection that are suitable for the construction of oligonucleotide sequences and a fluorescent probe for their use in real-time PCR. The search for primer sequences was carried out according to the following scheme: determination of the target gene and analysis of its variability, search for conserved regions and selection of optimal regions for primer design. According to the results of the research, the sequences flanking the 142 bp region were selected. Based on an in silico analysis of matrix primer correspondence and intraspecies specificity using FASTA on-line, suitability for the practical use of two primers and one probe for detection of chlamydia genetic material of different species was established

Keywords: chlamydiosis, diagnostics, PCR, primers

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