DOI: 10.36016/VM-2020-106-5

Veterinary Medicine: inter-departmental subject scientific collection. 2020. Issue 106. P. 24–28.

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STUDY OF BIOLOGICAL PROPERTIES OF BOVINE FOAMY VIRUS ON THE MODEL OF LABORATORY ANIMALS

Gorbatenko S. K. ¹, Solodiankin О. S. ¹, Gorbatenko V. P. ², Kovalenko L. V. ¹, Rudova N. G. ¹, Kuznetsova О. V. ¹, Miahkykh N. V. ¹, Zdanevych P. P. ¹

¹ National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine, e-mail: st.gorbatenko@gmail.com

² Kharkiv State Zooveterinary Academy, Kharkiv, Ukraine

Genetic material of the field isolate of bovine foamy virus was inoculated subcutaneously in rabbits (5 experimental and 5 intact animals). The influence of genetic material on the organism of experimental animals was studied at the molecular-genetic, cellular, and biochemical levels. It has been established that the persistence of the causative agent of spumavirus infection according to the results of molecular and genetic research (PCR) is 60 days. Redistribution of cells of leukocyte fraction towards lymphocytosis (80–88%) was recorded. Decrease in the concentration of circulating immune complexes by 22.2% (p ≤ 0.05) and a tendency to decrease in the seromucoid concentration (by 6.5%) were found on 60^th day after infection compared with control indicators. At the end of the experiment it was established a statistically significant decrease in the concentration of circulating immune complexes and an increase in seromucoid level by 21.5% and 17.6% respectively, as well as a tendency to decrease in the level of globulins, which was 15.5%. The results of hematological and biochemical analysis indicate the development of immunosuppressive state under the influence of the inoculated material

Keywords: PCR, hematological and biochemical indicators, immunosuppression

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