DOI: 10.36016/VM-2020-106-4

Veterinary Medicine: inter-departmental subject scientific collection. 2020. Issue 106. P. 19–23.

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HEMAGGLUTINATING PROPERTIES OF BOVINE PARAINFLUENZA-3, CORONAVIRUS AND ROTAVIRUS INFECTIONS’ PATHOGENS IN CATTLE

Kornieikov O. M., Borodai N. I., Oleshko A. Y., Perfilova S. I.

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine, e-mail: korneykov@ukr.net

Al Jabari Munir

Kharkiv State Zooveterinary Academy, Kharkiv, Ukraine

The purpose of the work was to determine the hemagglutinating activity of different strains of bovine parainfluenza-3, coronavirus and rotavirus infections’ pathogens in cattle. Accumulation of hemagglutinins of viruses was carried out by infecting cell cultures with viruses, determining their infectious activity by cytopathic action, followed by the establishment of hemagglutinating activity with erythrocytes of different species of animals. It has been established that within one type of virus there may be strains that have different hemagglutinating activity. It has been proved that within one type of virus there may be strains that have different hemagglutinating activity. It has been established that for the detection of coronavirus hemagglutinins mouse erythrocytes are the most suitable, bovine parainfluenza-3 virus hemagglutinins — guinea pig erythrocytes, rotavirus hemagglutinins — rooster erythrocytes. The relationship between the infectious activity of parainfluenza-3 virus, corona-, rotaviruses and their hemagglutinating properties has been established — the highest hemagglutinin titer was observed under the conditions of infectious activity of bovine parainfluenza-3 virus, corona-, rotaviruses in 7.0 lg TCD₅₀/cm³ and higher. Long-term storage of bovine parainfluenza-3, coronavirus and rotavirus infections’ pathogens at a temperature of minus 18 °С and lower did not have a negative effects on their hemagglutinating properties, in contrast to the storage of these viruses at a temperature of minus 4 °С or repeated defrosting

Keywords: infectious activity, cytopathic action, pneumoenteritis

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