DOI: 10.36016/VM-2020-106-2

Veterinary Medicine: inter-departmental subject scientific collection. 2020. Issue 106. P. 9–14.

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STUDY OF BIOLOGICAL PROPERTIES OF BRUCELLA OVIS STRAINS ISOLATED ON THE TERRITORY OF UKRAINE DURING 1973–2019

Marchenko N. V., Lymanska O. Yu., Kutsenko V. A., Gerilovych A. P., Bolotin V. I.

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine, e-mail: marcenkonata@gmail.com

The article presents data on the biological properties of Brucella strains, which were kept in the collection of microorganisms of NSC ‘IECVM’ previously and determined as Brucella ovis by biochemical tests. It was found that during long-term storage strains did not lose their properties according to passports. Four strains 78/3131, 157/4151, 169/87, and 68/Ж grew not only in the presence of thionine, which is characteristic of R-forms, but also in the media with fuchsin (1:50,000 and 1:100,000). When studying the antigenic properties, it was found that these strains have Brucella S-antigen and the absence of R-antigen. Additionally, molecular genetic typing revealed that four strains belonged to other species of Brucella

Keywords: ovine infectious epididymitis, PCR

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